This guide was made specifically for Leica programs and microscopes. The microscope we used to create this how-to guide is the Leica M205 C, consisting of z-stacking capabilities and a motorized column for effortless imaging.
Understanding the LAS X Software
The arrow box on the very left is a ‘Software Autofocus’ button.
The ‘Live Image’ button can be used to view what is currently under the microscope.
This button takes a Single Capture Image (i.e., not z-stacked) with all overlays stamped on it.
This button starts a Z-stack.
See Z-stacking settings section for more information.
On the top left side of the screen, you should see 6 boxes with icons.
Explorer: Organizes all the folders in the computer; where all of the photos taken on the program can be accessed.
Image: Where the exposure, gain, color mode, contrast, high dynamic range (HDR), and sharpening can be manipulated.
Illumination: Where the intensity of the lights on the microscope can be manipulated, in this case the SLI (Gooseneck) Light and HDI Light.
Magnification: Where the zoom and Iris control can be manipulated.
Z-stack: Where the z-stacked images are taken.
Autofocus: Where the digital autofocus system can be manipulated (not applicable).