This guide was made specifically for Leica programs and microscopes. The microscope we used to create this how-to guide is the Leica M205 C, consisting of z-stacking capabilities and a motorized column for effortless imaging.
Lighting
If that does not fix the problem, the next route to address is the lighting. Too dark or too bright a light setting can make it difficult for the camera to pick up on the specimen’s surface details, leaving parts unfocused.
The first step is to adjust the light intensity, which can be done on the scope or in the Illumination Tab in Leica (see Section 2).
Use the + and – buttons to control the light intensities. There are two main lights: the Dome Light (bottom rectangle) and the Objective Light (top rectangle).
You may also use the gooseneck lights to add contrast. Be aware that these lights add shadows.
If that does not fix the problem, start adjusting settings in the LAS X program. This can be done under the Image tab (see Section 2). If Auto-Exposure is turned on, try turning it off and manually adjusting the exposure.