This guide was made specifically for Leica programs and microscopes. The microscope we used to create this how-to guide is the Leica M205 C, consisting of z-stacking capabilities and a motorized column for effortless imaging.
Number Of Steps
If the lighting does not fix the problem, increase the ‘Number of Steps’ in the Z-stacking tab. When Z-stacking, the microscope will take more images of the specimen, which could help capture the areas that are unfocused. Start by adding 5-10 more steps than were showing under ‘System Optimized’.