This guide was made specifically for Leica programs and microscopes. The microscope we used to create this how-to guide is the Leica M205 C, consisting of z-stacking capabilities and a motorized column for effortless imaging.
Textured Seeds
Some seeds are very textured; with grooves, spikes or bumps. Some examples include Argemone sp. that have honeycomb-like dimples across the seed, or in Sanicula sp. that have burrs protruding from the surface.
To image these seeds, you may need to increase the ‘Number of Steps’ when z-stacking (see Section 3 – Number of Steps). This will allow the entire focal range of the seed to be represented.
You should also make sure that you the Beginning and End of your z-stack is set at the right focus range (see Section 3: Focus Range).