Viola chamissoniana subsp. tracheliifolia is a branched plant featuring short lateral branches and a taproot system. The stems may root along the lower parts when decumbent and are either glabrous or pubescent. The leaves are alternate, predominantly clustered towards the upper part of the branch, and exhibit a variety of shapes from deltate–ovate to deltate–cordate, typically measuring 5–29 cm in length and (2–)2.5–9 cm in width. These leaves have a long-acuminate or acuminate apex, a base ranging from truncate to cordate, and can be either glabrous or bear pubescence on the lower surfaces. The margins are dentate–serrate, and the petioles range from (0.6–)1.5–8 cm in length. The stipules are asymmetrically broad, deltate, and measure 5–16 mm by 2–7 mm, with remotely glandular–toothed and somewhat irregularly lacerate margins near the apex. Flowers of this subspecies are generally solitary per peduncle, axillary on stems and lateral branches, with peduncles measuring 8–60 mm and pedicels 20–70 mm in length. The flowers are bisexual and primarily chasmogamous, featuring a calyx of five subequal, linear-lanceolate sepals and a zygomorphic corolla composed of five distinct, unequal petals white with a purple tinge. Stamens measure 4.2–4.5 mm, with the connective of the lower ones extended into a unique structure, and the ovary is superior, 3-carpellate, and 1-celled. The fruit is a capsule with loculicidal dehiscence, and the seeds are numerous, dark brown to nearly black, obovoid, and measure 2.0–2.5 mm in length. The seed coat is somewhat leathery and dull, and the apex is rounded, with fleshy, oily endosperm. The plant typically grows in mesic to wet forest habitats between 330-1,890 meters.